Simple culture medium and method for preparation thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/00 (2006.01) C12N 1/20 (2006.01) C12N 1/22 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2374020

A simple culture medium produced by impregnating a fibrous water-absorbent sheet with a suspension formed by suspending in an alcohol (a) an adhesive (0.01-0.4 wt.%) which is soluble in both water and alcohol, (b) a gelling agent which is soluble in water and insoluble in alcohol, and (c) a bacterial nutritive ingredient, the fibrous water--absorbent sheet having a mesh larger than the particle size of the gelling agent and being placed on a waterproof flat plate, and by drying the resultant sheet while suppressing rapid evaporation of the alcohol, to thereby cause the water--absorbent sheet to adhere onto the waterproof flat plate; and a method for producing the medium.

L'invention concerne un milieu de culture simple que l'on obtient en apportant une suspension dans un alcool contenant a) de 0,01 à 0,4 % en poids d'un adhésif hydrosoluble et un alcool, b) un agent gélifiant hydrosoluble et insoluble dans un alcool et c) des composants alimentaires pour des bactéries, en imprégnant une feuille fibreuse, absorbant l'eau et présentant un treillis plus large que celui de l'agent gélifiant qui se trouve sur une plaque imperméable avec la suspension d'alcool. Puis on sèche la plaque obtenue avec la feuille tout en supprimant l'évaporation rapide de l'alcool pour lier la feuille absorbant l'eau à la plaque imperméable. L'invention concerne aussi un procédé de préparation du milieu de culture simple.

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