Isolation of membrane bound ligand-specific complexes

Details Isolation of membrane bound ligand-specific complexes Isolation of membrane bound ligand-specific complexes

C - Chemistry, Metallurgy

07

K

C07K 17/00 (2006.01) C07K 1/04 (2006.01) C07K 14/705 (2006.01) C07K 14/715 (2006.01) C07K 14/72 (2006.01) C07K 14/74 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2454133

A method to recover or "deracinate" receptors from intact membranes in their native configuration without removing microenvironmental components associated with them is described. The membranes containing the receptors are first associated with ligand-coupled solid supports to achieve association of the membranes through a receptor/ligand complex which includes the microenvironmental to the solid support and then removing the membrane from the receptor and its microenvironment by shear forces. The thus isolated receptors and their microenvironments can then be analyzed for their role in cellular differentiation, apoptosis, transformation and the like.

L'invention concerne une méthode permettant de récupérer ou de <=déraciner >= des récepteurs provenant de membranes intactes sous leur forme d'origine, sans retirer les composés microenvironnementaux qui y sont liés. Les membranes contenant les récepteurs sont, d'abord associées à des supports solides couplés aux ligands, de manière à parvenir à une association des membranes par le biais d'un complexe récepteur-ligand qui incorpore le microenvironnement au support solide et, puis, la membrane est retirée du récepteur et de son microenvironnement par l'intermédiaire de forces de cisaillement. On peut analyser les récepteurs ainsi isolés et leurs microenvironnements pour le rôle qu'ils jouent dans la différenciation, l'apoptose, la transformation cellulaires, et similaire.

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