Polynucleotides encoding choline monooxygenase and plants...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/53 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/04 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2276612

A full length choline monooxygenase (CMO) cDNA was cloned from spinach and used to transform plants which do not naturally express CMO. A method is presented to improve stress tolerance of crops following engineering of CMO and BADH in plants that lack glycine betaine accumulation. Also provided are fragments useful as probes to isolate other CMO-type genes, and antisense sequences which inhibit the production of CMO. Reduction of glycine betaine as a consequence of antisense expression of CMO in species naturally accumulating glycine betaine improves the transgenic plant's tolerance toward pathogens and pests and/or enhances its nutritional quality.

L'invention porte sur un ADN-c pleine longueur de choline-monooxygénase (CMO) cloné d'un épinard et utilisé pour modifier des plantes n'exprimant pas naturellement CMO. On décrit une méthode pour améliorer la tolérance au stress des plantes après manipulation de CMO et de BADH chez des plantes qui n'accumulent pas la bétaïne de glycine. L'invention porte également sur des fragments convenant comme sondes pour isoler d'autres gènes de type CMO et sur des séquences antisens qui inhibent la production de CMO. La diminution de la bétaïne de glycine résultant de l'expression antisens de CMO chez des espèces accumulant naturellement la bétaïne de glycine améliore la résistance d'une plante transgénique à des agents pathogènes ou ennemis des plantes et/ou accroît sa valeur nutritionnelle.

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