Protein expression systems for non-pathogenic kinetoplastidae

Details Protein expression systems for non-pathogenic kinetoplastidae Protein expression systems for non-pathogenic kinetoplastidae

C - Chemistry, Metallurgy

12

N

C12N 15/79 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2388151

The object of the present invention is a method of recombinant protein production in vitro with cultivated non pathogenic Kinetoplastidae parasites (Leishmania tarentolae, Crithidia fasciculata, Wallaceina inconstans) (former Proteomonas inconstans), (Leptomonas collos, Leptomonas sp. Cfm, Leptomonas sp. Nfm or Leptomonas seymouri). This includes description of cultivation conditions for several species that allow large scale growth of these organisms on cheap media. The method of the invention includes strategies and construct description for generation of stably transfected organisms capable of expression of recombinant proteins.

La présente invention concerne un procédé de production <i>in vitro</i> de protéines recombinées avec des parasites <i>Kinetoplastidae</i> non pathogènes de culture (<i>Leishmania tarentolae</i>, <i>Crithidia fasciculata</i>, <i>Wallaceina inconstans</i>) (ancien <i>Proteomonas inconstans</i>), (<i>Leptomonas collos, Leptomonas sp. Cfm, Leptomonas sp. Nfm</i> ou <i>Leptomonas seymouri</i>). Ceci comprend la description de conditions de cultures pour différentes espèces pour permettre la croissance à grande échelle de ces organismes sur un milieu à bas prix. Le procédé de l'invention comprend des stratégies et la description de gènes pour la production d'organismes transfectés stables capables d'exprimer des protéines recombinées.

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