Diagnosis, staging and monitoring of inflammatory bowel disease

Details Diagnosis, staging and monitoring of inflammatory bowel disease Diagnosis, staging and monitoring of inflammatory bowel disease

G - Physics

01

N

G01N 33/68 (2006.01) C07K 14/525 (2006.01) C07K 14/54 (2006.01) C07K 14/57 (2006.01) C07K 14/705 (2006.01) C07K 14/72 (2006.01)

Patent

CA 2695360

A method of differentiating between active and inactive IBD in a gastrointestinal mucosa sample or a sample from a sentinel lymph node draining gastrointestinal mucosa comprises preparing a suspension of single cells from the sample, analyzing the suspension for expression of the inflammation activation marker CD69 on CD4+ T helper cells using directly labelled fluorescent DC69 antibody; comparing the number of T helper cells expressing DC69 in the sample with that obtained from a corresponding sample of a healthy person, a significantly increased level of T helper cells expressingCDD69 signifying the presence of active IBD and a less than significantly increased level of T helper cells signifying the presence of inactive IBD. Also disclosed are methods of differentiating between ulcerative colitis (UC) and Crohn's disease (CD), of detecting UC and CD, and of determining the susceptibility of an IBD patient to steroid treatment.

L'invention porte sur un procédé de différenciation entre des affections abdominales inflammatoires (IBD) actives et inactives dans un échantillon de muqueuse gastro-intestinale ou un échantillon provenant d'un ganglion lymphatique sentinelle drainant la muqueuse gastro-intestinale. Ce procédé comporte la préparation d'une suspension de cellules individuelles provenant de l'échantillon, l'analyse de la suspension pour l'expression du marqueur d'activation d'inflammation CD69 sur des lymphocytes T auxiliaires CD4+ à l'aide d'un anticorps CD69 fluorescent directement marqué; la comparaison du nombre de lymphocytes T auxiliaires exprimant CD69 dans l'échantillon avec celui obtenu à partir d'un échantillon correspondant d'une personne en bonne santé, un taux significativement accru de lymphocytes T auxiliaires exprimant CD69 signifiant la présence d'une IBD active et un taux moins que significativement accru de lymphocytes T auxiliaires signifiant la présence d'une IBD inactive. L'invention porte également sur des procédés de différenciation entre une colite ulcérative (UC) et la maladie de Crohn (CD), de détection de UC et CD et de détermination de la sensibilité d'un patient atteint d'une IBD à un traitement stéroïdien.

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