Sucrose biosensors and methods of using the same

Details Sucrose biosensors and methods of using the same Sucrose biosensors and methods of using the same

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A01H 5/00 (2006.01) A01K 67/027 (2006.01) A61K 49/00 (2006.01) C07K 14/195 (2006.01) C07K 19/00 (2006.01) C12N 15/31 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2625165

Sucrose biosensors are disclosed, which comprise a sucrose binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon sucrose binding. Such biosensors are useful for real time monitoring of sucrose metabolism in living cells.

L'invention concerne des biocapteurs de saccharose comprenant un domaine de fixation du saccharose combiné avec des groupes donneurs et fluorescents, permettant de détecter et de mesurer le transfert d'énergie de résonance par fluorescence lors de la fixation du saccharose. Ces biocapteurs sont utiles pour le contrôle en temps réel du métabolisme du saccharose dans des cellules vivantes.

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