Vaccine against microbial pathogens

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/02 (2006.01) A61K 39/04 (2006.01) A61P 31/04 (2006.01)

Patent

CA 2421229

The present invention provides a vaccine comprising a microbial pathogen, wherein the microbial pathogen is subjected to a stress inducing stimuli. The stress inducing stimuli can be heat or osmotic stress, through preferably the microbial pathogen is genetically modified such that at least one repressor gene for a heat shock protein gene is inactived, this allowing the constitutive expression of heat shock proteins. In particular the use of heat shock protein repressor mutant bacteria is shown to be effective for inducing immunity when comprised within vaccines of the present invention. The present invention further provides a method for producing a vaccine comprising stressed induced microbial pathogens and further the use of the heat shock protein repressor deletion mutant microbes as vaccine vectors which can be additionally allow the expression of heterologous antigen fragments.

L'invention concerne un vaccin comprenant un microbe pathogène, ce microbe pathogène étant soumis à une sollicitation induisant des stimuli. Cette sollicitation induisant des stimuli peut être une sollicitation thermique ou un stress osmotique, bien que de préférence le microbe pathogène soit modifié génétiquement de manière qu'au moins un gène répresseur pour un gène de protéine de choc thermique soit inactivé, ceci permettant ainsi l'expression constitutive de protéines de choc thermique. Plus spécifiquement, on montre que l'utilisation d'une bactérie mutante de répresseur de protéine de choc thermique est efficace dans l'induction de l'immunité lorsqu'elle est contenue dans les vaccins de cette invention. L'invention concerne également un procédé de production d'un vaccin comprenant des microbes pathogènes induits soumis à sollicitation et également l'utilisation de microbes mutants de délétion de répresseur de la protéine de choc thermique en tant que vecteurs de vaccin pouvant permettre en outre l'expression de fragments antigènes hétérologues.

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