Mixed haptenized tumor cells and extracts and methods of...

A - Human Necessities – 61 – K

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A61K 35/00 (2006.01) A61K 35/12 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2474960

This invention relates to compositions comprising multi-haptenized tumor cells and extracts thereof, methods for preparing the compositions, vaccines comprising such multi-haptenized tumor cells, and methods for treating cancer with such vaccines. In a specific embodiment, melanoma and ovarian adenocarcinoma cells are multi-haptenized, wherein the tumor cells are differentially haptenized either with a dinitrophenyl group coupled to .epsilon.-amino groups, or with a sulfanilic acid group coupled to aromatic side chains of histidine and tyrosine. A method of SA-haptenization is also provided.

L'invention concerne des compositions contenant des cellules tumorales multi-hapténisées ou des extraits de celles-ci, des procédés de préparation de ces compositions, des vaccins contenant de telles cellules tumorales multi-hapténisées, ainsi que des méthodes de cancérothérapie faisant intervenir ces vaccins. Dans un mode de réalisation spécifique, les cellules de mélanomes et d'adénocarcinomes ovariens sont multi-hapténisées ; les cellules tumorales étant hapténisées de façon différentielle soit avec un groupe dinitrophényle couplé à des groupes e-amino, soit avec un groupe d'acide sulfanilique couplé à des chaînes latérales aromatiques de histidine et tyrosine. L'invention concerne en outre un procédé d'hapténisation par SA.

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