Protein expression system

Details Protein expression system Protein expression system

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 1/22 (2006.01) C07K 14/31 (2006.01) C07K 14/315 (2006.01) C07K 14/47 (2006.01) C07K 14/475 (2006.01) C07K 14/48 (2006.01) C07K 14/735 (2006.01) C07K 19/00 (2006.01) C12N 9/64 (2006.01) C12N 15/74 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2245006

The present invention relates to improved recombinant vectors which allow for the production of fusion proteins. The present invention also relates to methods for the expression and purification of authentic recombinant proteins from such fusion proteins. In particular, the present invention relates to fusion proteins wherein additional domains and/or elements are added to the fusion proteins. Included in these domains and/or elements are Fc fragments (1) fused to proteins of interest (2) by a polypeptide comprising a hinge region (3), hydrophilic spacer (4), and a dibasic amino acid endoprotease cleavage site (5), wherein the spacer may be cleaved and then digested by carboxypeptidase B (6) to yield the authentic protein (2).

Vecteurs de recombinaison améliorés permettant de produire des protéines de fusion. L'invention porte également sur des méthodes d'expression et de purification de protéines authentiques de recombinaison à partir de telles protéines de fusion. Elle porte en particulier sur des protéines de fusion dans lesquelles les domaines additionnels et/ou les éléments additionnels sont ajoutés à ces protéines. Dans ces domaines et/ou éléments, sont inclus des fragments Fc (1) ayant subi une fusion avec ces protéines (2) grâce à un polypeptide comprenant une région charnière (3), un espaceur hydrophile (4) et un site de clivage d'endoprotéase amino acide dibasique (5), l'espaceur pouvant être clivé, puis digéré par la carboxypeptidase B (6) pour produire une protéine authentique (2).

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