Process for purifying proteins

Details Process for purifying proteins Process for purifying proteins

C - Chemistry, Metallurgy

07

K

C07K 1/20 (2006.01)

Patent

CA 2554929

The invention relates to a process for purifying a protein by mixing a protein preparation with a solution having a first salt and a second salt, wherein each salt has a different lyotropic value, and loading the mixture onto a hydrophobic interaction chromatography column. The dynamic capacity of the column for a protein using the two salt combination will be increased compared with the dynamic capacity of the column for either single salt alone.

L'invention concerne un procédé destiné à purifier une protéine par mélange d'une préparation de protéines avec une solution possédant un premier et un second sel, chaque sel présentant une valeur lyotropique différente, et par chargement du mélange dans une colonne chromatographique à interaction hydrophobe. La capacité dynamique de la colonne pour une protéine utilisant la combinaison des deux sels peut être augmentée par rapport à la capacité dynamique de la colonne utilisant l'un de deux sels uniquement.

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