Use of dna oligomers for inhibition of hiv by decreasing...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) A61K 31/70 (2006.01) C07H 21/00 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2186107

The present invention relates to antisense DNA oligomers which are complementary to regions of the RNA of the small ribosomal subunit of mammalian cells, which are selected from the group consisting of: a) nucleotides 1 to 20 in human 18S rRNA corresponding to region 10 in E. coli 16S rRNA; b) nucleotides 595 to 641 in human 18S rRNA corresponding to region 530 in E. coli 16S rRNA; c) nucleotides 1182 to 1220 in human 18S rRNA corresponding to region 900 in E. coli 16S rRNA; and d) nucleotides 1695 to 1715 in human 18S rRNA corresponding to region 1400 in E. coli 16S rRNA; whereby, said oligomers decrease the occurrence of ribosomal frameshifting and inhibit the expression of viral enzymatic proteins. I

L'invention concerne des oligomères d'ADN antisens qui sont complémentaires de régions de l'ADN de la petite sous-unité ribosomique de cellules de mammifères, et qui sont choisis dans le groupe consistant en: a) les nucléotides 1 à 20 de ARN ribosomique humain de 18 S, correspondant à la région 10 de l'ARN ribosomique de 16S d'E. coli; b) les nucléotides 595 à 641 de l'ARN ribosomique humain correspondant à la région 530 de l'ARN ribosomique de 16S d'E. coli; les nucléotides 1182 à 1220 de l'ARN ribosomique humain de 18S, correspondant à la région 530 de l'ARN ribosomique de 16S d'E. coli; les nucléotides 1182 à 1220 de l'ARN ribosomique humain de 18S, correspondant à la région 900 de l'ARN ribosomique de 16S d'E. coli; et les nucléotides 1695 à 1715 de l'ARN ribosomique humain de 18S, correspondant à la région 400 de l'ARN ribosomique de 16S d'E. coli. Ces oligomères diminuent l'apparition du décalage de trame ribosomique et inhibent l'expression des protéines enzymatiques virales.

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