Multiple assay method

G - Physics – 01 – N

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Details

G01N 33/58 (2006.01) C12Q 1/00 (2006.01) G01N 33/543 (2006.01) G01N 15/14 (2006.01)

Patent

CA 2313047

A method for the assay of N samples each containing a compound to be tested, comprises providing N reaction vessels each containing a population of carrier beads and other reagents for performing the assay, where N is at least 2 e.g. 80-4000. Each population of carrier beads is distinguishable from every other population. After adding the samples to the reaction vessels and performing the assays, the contents of all the reaction vessels are mixed and subjected to analysis by flow cytometry. By means of flow cytometry, each carrier bead is rapidly analysed to identify its population and also to determine the presence or concentration or biological activity of the compound to be tested.

Procédé de dosage de N échantillons contenant chacun un composé à analyser, qui consiste à disposer de N récipients de réaction contenant chacun une population de perles de support et d'autres réactifs permettant d'effectuer le dosage, N étant au moins 2, par exemple 80 à 4000. Chaque population de perles de support peut être distinguée de chacune des autres populations. Une fois les échantillons ajoutés aux récipients de réaction et les réactions effectuées, les contenus de tous les récipients de réaction sont mélangés et soumis à l'analyse par la cytométrie de flux. Cette technique permet d'analyser rapidement chaque perle de support pour identifier sa population et également déterminer la présence ou la concentration ou l'activité biologique du composé à analyser.

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