Hsv/aav hybrid amplicon vectors

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) A01N 43/04 (2006.01) A61K 31/70 (2006.01) C07K 14/015 (2006.01) C12N 15/869 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2271777

The present invention relates to a hybrid vector system which incorporates elements of herpesvirus and adeno-associated virus which are capable of expressing a gene product in eukaryotic cells. The vector system of the present invention provides a means of packaging plasmid DNA into highly infectious virions which can efficiently and safely deliver large transgenes to both mitotic and postmitotic cells in a state which can be maintained for extended periods. The invention pertains to the use of this vector system in introducing and expressing gene sequences in mitotic and postmitotic cells for therapeutic purposes.

La présente invention se rapporte à un système de vecteurs hybrides qui comprend des éléments d'herpèsvirus et de virus adéno-associé, susceptibles d'exprimer un produit génique dans des cellules eucaryotes. Le système de vecteurs de la présente invention constitue un moyen d'encapsidation d'ADN plasmidique au sein de virions extrêmement infectieux qui peuvent délivrer, de manière sure et efficace, de grands transgènes à la fois à des cellules mitotiques et à des cellules post-mitotiques, dans un état qui peut être maintenu pendant des périodes prolongées. Cette invention se rapporte à l'utilisation de ce système de vecteurs pour introduire et exprimer des séquences géniques dans des cellules mitotiques et post-mitotiques à des fins thérapeutiques.

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