Cell/tissue analysis via controlled electroporation

Details Cell/tissue analysis via controlled electroporation Cell/tissue analysis via controlled electroporation

C - Chemistry, Metallurgy

12

N

C12N 13/00 (2006.01) C12M 3/00 (2006.01) G01N 33/487 (2006.01)

Patent

CA 2378110

An electrical current is created across an electrically conductive medium comprising a cell which may be part of a tissue of a living organism. A first electrical parameter which may be current, voltage, or electrical impedance is measured. A second electrical parameter which may be current, voltage or a combination of both is then adjusted and/or analyzed. Adjustments are carried out to facilitate analysis and/or obtain a desired degree of electroporation. Analysis is carried out to determine characteristics of the cell membrane and/or tissue.

L'invention concerne un courant électrique créé sur un support conducteur qui comporte une cellule pouvant faire partie d'un tissu ou d'un organisme vivant. On mesure un premier paramètre électrique, tel qu'un courant, une tension, ou une impédance électrique. On règle et/ou on analyse ensuite un second paramètre électrique, tel qu'un courant ou une tension ou une combinaison des deux. Des règlements sont effectués afin de faciliter l'analyse et/ou d'obtenir le dégré d'électroporation souhaité. On procède ensuite à une analyse afin de déterminer des caractéristiques de la membrane cellulaire et/ou du tissu.

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