Purification of factor viii using a mixed-mode or multimodal...

Details Purification of factor viii using a mixed-mode or multimodal... Purification of factor viii using a mixed-mode or multimodal...

C - Chemistry, Metallurgy

07

K

C07K 1/16 (2006.01) C07K 14/755 (2006.01)

Patent

CA 2692814

A method for purifying a recombinant protein using a multimodal or mixed mode resin containing ligands which comprise a hydrophobic part and a negatively charged part is described. The invention is advantageous in that it is a single step chromatographic process which does not require adjustment of pH or conductivity during loading step and results in high yield and potency. The process is used for the purification of recombinant compositions of coagulation factor, particularly recombinant Factor VIII.

L'invention porte sur un procédé de purification d'une protéine recombinante à l'aide d'une résine multimodale ou en mode mixte contenant des ligands qui comprennent une partie hydrophobe et une partie chargée négativement. L'invention est avantageuse par le fait que c'est un procédé chromatographique à une seule étape qui ne nécessite pas d'ajustement de pH ou de conductivité pendant l'étape de charge et conduit à rendement élevé et une puissance élevée. Le procédé est utilisé pour la purification de compositions recombinantes de facteur de coagulation, en particulier le Facteur VIII recombinant.

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