Method of synthesizing saccharide compositions

C - Chemistry – Metallurgy – 12 – P

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C12P 21/00 (2006.01) C12M 1/40 (2006.01) C12N 9/10 (2006.01) C12N 15/31 (2006.01) C12P 19/00 (2006.01) C12P 19/18 (2006.01)

Patent

CA 2167157

A method of synthesizing saccharide compositions is described. In this method, an acceptor moiety is contacted with at least one donor saccharide in the presence of at least one cell surface-bound glycosyltransferase specific for catalyzing the coupling of the acceptor moiety with the donor saccharide. The acceptor moiety used is a carbohydrate, a protein, a glycoprotein a lipid, or a glycolipid.

Procédé de synthèse de compositions de saccharide. Selon ce procédé, une fraction accepteur est mise en contact avec au moins un saccharide donneur en présence d'au moins une glycosyltransférase liée à la surface cellulaire et apte à catalyser de manière spécifique le couplage de la fraction accepteur et du saccharide donneur. La fraction accepteur utilisée est un glucide, une protéine, un glycolipide, un lipide ou une glycoprotéine.

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