Promoters from plant protoporphyrinogen oxidase genes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2247797

Promoters naturally associated with plant protoporphyrinogen oxidase (protox) coding sequences, and derivatives thereof, are provided. These promoters can be used to control the expression of an operably linked heterologous coding sequence in a plant cell. These promoters are particularly useful for expressing modified forms of herbicide target enzymes, particularly modified forms of protox, to achieve tolerance to herbicides that inhibit the corresponding unmodified enzymes. Recombinant DNA molecules and chimeric genes comprising these promoters are provided, as well as plant tissue and plants containing such chimeric genes.

Cette invention concerne des promoteurs qui sont naturellement associés à des séquences codant l'oxydase protoporphyrinogène (protox) d'origine végétale, ainsi que des dérivés de ces promoteurs. Ces promoteurs peuvent être utilisés afin de gérer l'expression d'une séquence de codage hétérologue et à liaison fonctionnelle dans une cellule végétale. Ces promoteurs sont particulièrement utiles dans l'expression de formes modifiées d'enzymes cibles herbicides, notamment l'expression de formes modifiées de protox, et permettent ainsi d'obtenir une tolérance aux herbicides qui inhibent les enzymes non modifiées correspondantes. Cette invention concerne également des molécules d'ADN recombinant et des gènes chimériques contenant ces promoteurs, ainsi que des tissus végétaux et des plantes contenant de tels gènes chimériques.

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