Mammalian expression vector

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2745311

The present invention describes new mammalian expression vectors comprising a novel combination of regulatory elements and one or more selection marker gene(s). The vector allows for incorporation of at least one, preferably two or more genes of interest, its/their subsequent expression, and for selection of transfected cells using, e.g., G418 and/or MTX. The pDGP.DELTA.GOI vector as an example for a mammalian expression vector according to the present invention exhibits a 9555 bp sequence, one strand of which is represented by SEQ ID NO:2.

La présente invention concerne de nouveaux vecteurs d'expression de mammifère comprenant une nouvelle combinaison d'éléments de régulation et un ou plusieurs gènes marqueurs de sélection. Le vecteur permet l'incorporation d'au moins un gène d'intérêt, de préférence de deux ou plus de deux gènes d'intérêt, son/leur expression ultérieure, et la sélection des cellules transfectées au moyen, par exemple, de G418 et/ou de MTX. Le vecteur pDGP?GOI, qui est un exemple de vecteur d'expression de mammifère selon la présente invention, contient une séquence de 9555 pb, dont un brin est représenté par SEQ ID NO:2.

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