Epigenetic methods

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2689537

The present invention provides methods for obtaining epigenetic information for a polyploid subject, the method including the steps of obtaining a biological sample from the subject, the sample containing: (i) at least one paternally- derived DNA molecule and/or associated protein and/or, (ii) at least one maternally-derived DNA molecule and/or associated protein, analyzing any one or more of the paternally- or maternally-derived DNA molecules or associated proteins for the presence or absence of modifications, wherein the step of analyzing determines whether any two modifications are present in cis on one chromosome, or in trans across two sister chromosomes.

L'invention porte sur des méthodes d'obtention d'informations épigénétiques sur un sujet de polyploïde, la méthode comporte les étapes suivantes: obtention d'un échantillon biologique du sujet contenant : (i) au moins une molécule d'ADN d'origine paternelle et-ou la protéine associée et-ou, (ii) au moins une molécule d'ADN d'origine maternelle et-ou la protéine associée; analyse d'une ou des molécules d'ADN d'origine paternelle ou maternelle ou des protéines associées pour y détecter la présence ou l'absence de modifications l'analyse, et déterminer si deux modifications sont présentes en cis sur un chromosome ou en trans sur deux chromosome frères.

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