Method for analysing nucleic acids

G - Physics – 01 – N

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G01N 27/447 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2417534

The invention concerns compositions and methods for analysing, separating or sequencing nucleic acids. More particularly, it concerns methods for analysing, separating or sequencing nucleic acids by electrophoresis, in particular capillary, more preferably gel-free. The invention also concerns methods for treating nucleic acids, or separating nucleic acids, applicable to rapid and efficient sequencing. The invention further concerns products, compositions and kits for implementing said methods. The invention consists particularly in the use of a positively charged carrier molecule, enabling to efficiently separate nucleic acids in solution. The invention is useful for analysing, separating or sequencing nucleic acids of different type and origin, in the field of experiment, diagnosis, medicine and the like, in particular denovo gene typing and sequencing.

La présente invention concerne des compositions et méthodes pour l'analyse, la séparation ou le séquençage d'acides nucléiques. Elle concerne plus particulièrement des méthodes d'analyse, de séparation ou de séquençage d'acides nucléiques par électrophorèse, notamment capillaire, plus préférentiellement sans gel. La présente invention concerne également des méthodes de traitement des acides nucléiques, ou de séparation d'acides nucléiques, applicables au séquençage rapide et efficace. L'invention réside également dans les produits, compositions et kits pour la mise en oeuvre de ces méthodes. L'invention repose notamment sur l'utilisation d'une molécule porteuse chargée positivement, permettant de séparer efficacement des acides nucléiques en solution. L'invention est applicable à l'analyse, la séparation ou le séquençage d'acides nucléiques de nature et d'origine variées, dans le domaine expérimental, diagnostic, médical, etc., notamment le génotype et le séquençage de novo.

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