Method of obtaining a product sugar stream from cellulosic...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/00 (2006.01) C12P 7/10 (2006.01)

Patent

CA 2572502

A process for obtaining a product sugar stream from cellulosic biomass is disclosed. In one process, the cellulosic biomass is pretreated at a pH between about 0.4 to 2.0 by adding one or more than one acid to produce a pretreated cellulosic biomass comprising acetic acid. One or more than one base is then added to the pretreated cellulosic biomass to adjust the pretreated cellulosic biomass to a pH of about 4.0 to about 6.0 to produce a neutralized cellulosic biomass comprising inorganic salt and acetate salt. The neutralized biomass is then hydrolyzed by cellulase enzymes to produce a crude sugar stream. Insoluble residue is separated from the crude sugar stream and the resulting clarified sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more raffinate streams and a product stream. The raffinate stream comprises inorganic salts and acetate salts, and the product stream comprises sugar. The product stream may then be fermented or otherwise further processed. In an alternate process, a product sugar stream is obtained from a crude sugar stream that is produced from conversion of cellulosic biomass to sugar. The cellulosic biomass may be produced using any suitable method. In this process the crude sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more than one raffinate stream comprising sulfate and acetate salts, and a product stream comprising sugar, and the product sugar stream is obtained.

L'invention concerne un procédé d'élaboration de flux de produit sucre à partir d'une biomasse cellulosique. Selon une variante, la biomasse est prétraitée à un pH compris entre environ 0,4 et 2 par adjonction d'un ou plusieurs acides, et la biomasse résultante comprend de l'acide acétique. On ajoute ensuite une ou plusieurs bases pour ajuster le pH entre environ 4 et environ 6, ce qui donne une biomasse neutralisée avec sel inorganique et sel acétate. Ensuite, la biomasse est hydrolysée par enzymes cellulases en flux de sucre brut. Le résidu insoluble est séparé de ce flux, et le flux résultant clarifié est traité en chromatographie par exclusion d'ions à un pH compris entre environ 5 et environ 10, ce qui donne un ou plusieurs flux de raffinat et un flux de produit. Le flux de raffinat comprend des sels inorganiques et des sels acétate, et le flux de produit comprend du sucre. Le flux de produit peut ensuite être fermenté ou subir un autre traitement. Selon une variante, un flux de produit sucre résulte du flux de sucre brut issu de la conversion de biomasse cellulosique en sucre. La biomasse cellulosique peut être produite selon un procédé approprié quelconque. Ainsi, le flux de sucre brut est traité en chromatographie par exclusion d'ions à un pH compris entre environ 5 et environ 10, ce qui donne un ou plusieurs flux de raffinat comprenant des sels sulfate et acétate, et un flux de produit comprenant du sucre, à savoir le flux de produit sucre recherché.

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