G - Physics – 01 – N
Patent
G - Physics
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N
G01N 33/542 (2006.01) G01N 33/53 (2006.01) G01N 33/535 (2006.01) G01N 33/536 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01) G01N 33/76 (2006.01)
Patent
CA 2079891
2079891 9115767 PCTABS00007 Reagents for a liquid-phase immunodiagnostic assay (LIDA) method comprise a first enzyme; a second enzyme; a first agent which is capable of binding with an analyte to form a complex, said agent being attached to one of said first and second enzymes; and a complex-binding agent attached to the remaining enzyme, wherein said first enzyme is capable of interacting with a substrate for said first enzyme together with any necessary additional substrates for said first enzyme to produce a substrate for said second enzyme, and wherein said second enzyme is capable of interacting with said substrate produced by said first enzyme together with any necessary additional substrates, such that occurrence of the second of said interactions is detectable. The reagent optionally further comprises a scavenger substance capable of inactivating the substrate produced by the first enzyme.
Fetherstonhaugh & Co.
University Of Florida
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