Methods for using resonance energy transfer-based assay of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/70 (2006.01) C12Q 1/02 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2178495

The subject invention provides methods for determining whether an agent is capable of either inhibiting or specifically inhibiting the fusion of CD4+cell with SSD HIV-1 envelope glycoprotein+cell. The subject invention also provides a method for determining whether an agent is capable of specifically inhibiting the infection of a CD4+cell with HIV-1 . This imvention also provides methods for quantitatively determining the ability of an antibody containing sample to either inhibit or specifically inhibit the fusion of CD4+cell with an HIV-1 envelope glycoprotein+cell.

L'invention concerne des procédés permettant de déterminer si un agent peut inhiber, de manière spécifique éventuellement, la fusion d'une cellule CD4+ avec une cellule de glycoprotéine+ d'enveloppe de VIH-1. Elle concerne aussi un procédé permettant de déterminer si un agent peut inhiber spécifiquement l'infection d'un CD4+ par le VIH-1. Elle concerne enfin des procédés qui permettent de déterminer quantitativement la capacité d'un échantillon contenant un anticorps d'inhiber, de manière spécifique éventuellement, la fusion d'un CD4+ avec une cellule de glycoprotéine+ d'enveloppe de VIH-1.

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