Nucleic acid for a cloning vector

Details Nucleic acid for a cloning vector Nucleic acid for a cloning vector

C - Chemistry, Metallurgy

12

N

C12N 15/66 (2006.01) C12N 15/10 (2006.01) C12N 15/65 (2006.01)

Patent

CA 2460505

The invention concerns a nucleic acid forming part of a cloning vector or designed to be inserted in a cloning vector. Said nucleic acid comprises at least a functional nucleotide sequence, which is needed for cloning and/or expression and is coupled with at least a sequence for identifying type II restriction endonucleases (REII), so that the cleavage point of the REII concerned is inside said functional nucleotide sequence. The invention also relates to a method using said nucleic acid.

L'invention concerne un acide nucléique faisant partie d'un vecteur de clonage ou conçu pour être inséré dans un vecteur de clonage. Cet acide nucléique comprend au moins une séquence nucléotidique fonctionnelle, qui est nécessaire au clonage et/ou à l'expression et est accouplée à au moins une séquence d'identification des endonucléases de restriction de type II (REII), de façon que le point de coupe des REII concernées se trouve à l'intérieur de ladite séquence nucléotidique fonctionnelle. L'invention se rapporte en outre à un procédé faisant appel à l'acide nucléique selon l'invention.

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