Microfluidic system for proteome analysis

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) B01L 3/00 (2006.01) C12M 1/40 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2464350

The invention provides a microfluidic system and method to rapidly analyze large number of compounds or complex mixtures of compounds, particularly, low abundance cellular proteins involved in cell signaling pathways. In one aspect, an integrated microfluidic system (1) comprises an upstream separation module (2) (preferably, a multi-dimensional separation device (2a)), a microfluidic device (5) for on-device protein digestion of substantially separated proteins received from the upstream separation module (2), a downstream separation module (14) for separating digestion products of said proteins, a peptide analysis module (17) and a processor (18) for determining the amino acid sequence of said proteins. Preferably, the integrated microfluidic system (1) comprises an interfacing microfluidic device (5i) between the downstream separation module (14) and the peptide analysis module (17).

L'invention concerne un système et un procédé microfluidiques permettant d'analyser rapidement un grand nombre de composés ou de mélanges complexes de composés, notamment des protéines cellulaires de faible abondance qui jouent un rôle dans les voies de signalisation cellulaire. Dans un aspect, un système microfluidique intégré (1) comprend un module de séparation en amont (2) (de préférence, un dispositif de séparation multidimensionnel (2a)), un dispositif microfluidique (5) servant à effectuer une digestion sur dispositif des protéines sensiblement séparées reçues du module de séparation en amont (2), un module de séparation en aval (14) servant à séparer les produits de digestion desdites protéines, un module d'analyse peptidique (17) et un processeur (18) servant à déterminer la séquence d'acide aminé desdites protéines. De préférence, le système microfluidique intégré (1) comprend un dispositif microfluidique d'interface (5i) entre le module de séparation en aval (14) et le module d'analyse peptidique (17).

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