Polynucleotide and its use for modulating a defence response...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2260363

The cloning of the Mlo gene of Barley, mlo mutants, and homologues from other species including rice and Arabidopsis thaliana enables manipulation of pathogen defence responses in plants. Nucleic acid and polypeptides are used in the production of transgenic plants in which the pathogen defence response is modulated, particularly stimulated. Various approaches enable diagnostic determination of the presence of susceptibility or resistance alleles in plants. Compounds able to modulate a defence response by interaction with Mlo or mlo protein are identifiable using assays involving the protein or fragments thereof.

Le clonage du gène Mlo de Barley, de mutants mlo, et d'homologues d'autres espèces comprenant le riz et l'Arabidopsis thaliana permet de manipuler des réponses de défense pathogène chez la plante. L'acide nucléique et les polypeptides sont utilisés dans la production de plantes transgéniques dans lesquelles la réponse de défense pathogène est modulée, et particulièrement stimulée. Diverses approches permettent de diagnostiquer la présence d'allèles de résistance ou de susceptibilité dans les plantes. Les composés permettant de moduler une réponse de défense par interaction avec la protéine Mlo ou mlo peuvent être identifiés par des analyses impliquant cette protéine ou des fragments de celle-ci.

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