Method for localization and quantitation of a substance in a...

Details Method for localization and quantitation of a substance in a... Method for localization and quantitation of a substance in a...

G - Physics

01

N

G01N 33/53 (2006.01) C12Q 1/68 (2006.01) G01N 21/63 (2006.01) G01N 33/535 (2006.01)

Patent

CA 2264762

A method for localizing and quantitating a target substance in a biological sample is disclosed. The method utilizes an enzyme-linked probe that binds to the target substance and generates a depositable photoluminescent substance which detects position and a soluble photoluminescent substance which allows quantitation in the medium bathing the sample.

On décrit un procédé de localisation et de dosage d'une substance cible dans un échantillon biologique. Ce procédé consiste à utiliser une sonde qui est liée à une enzyme, qui se fixe sur la substance cible et produit une substance photoluminescente pouvant se déposer et détectant la position de la substance cible, ainsi qu'une substance photoluminescente soluble permettant le dosage de la substance cible dans le milieu dans lequel baigne l'échantillon.

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