Medium for preparing dedifferentiated cells

Details Medium for preparing dedifferentiated cells Medium for preparing dedifferentiated cells

C - Chemistry, Metallurgy

12

N

C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2388208

The present invention relates to a medium for preparing dedifferentiated cells derived from post-natal islets of Langerhans. The medium comprises in a physiologically acceptable culture medium an effective amount of a solid matrix environment for a three-dimensional culture, a soluble matrix protein, and a first and a second factor for developing, maintaining and expanding the dedifferentiated cells. Such a medium may be used in an in vitro method for islet cell expansion.

La présente invention concerne un milieu servant à la préparation de cellules dédifférenciées dérivées d'îlots de Langerhans postnataux. Le milieu comprend, dans un milieu de culture acceptable d'un point de vue physiologique, une quantité efficace d'un environnement à matrice solide pour une culture tridimensionnelle, une protéine de matrice solide, et un premier et un second facteur servant au développement, au maintien, et à l'expansion des cellules dédifférenciées. Un milieu de ce type peut être utilisé dans le cadre d'un procédé d'expansion d'îlot in vitro.

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