Cho-k1 cell line

Details Cho-k1 cell line Cho-k1 cell line

C - Chemistry, Metallurgy

12

N

C12N 15/69 (2006.01) C07K 16/00 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2701677

The current invention reports a CHO-K1 cell, characterized in that said CHO-K1 cell is derived from CHO-K1 cell deposited as ATCC CCL-61, grows in suspension, requires no glutamine, no insulin, and no growth factors in the cultivation medium for growth, whereby said CHO-K1 cell is not modified compared to the deposited CHO-K1 cell ATCC CCL-61 cell line by the introduction, deletion, or inactivation of a nucleic acid. Also reported are a method for obtaining said CHO-K1 cell and a method for the production of a heterologous polypeptide using such a CHO-K1 cell according to the invention.

La présente invention concerne une cellule CHO-K1, caractérisée par le fait que ladite cellule CHO-K1 est issue d'une cellule CHO-K1 déposée en tant que ATCC CCL-61, se développe en suspension, ne nécessite pas de glutamine, pas d'insuline ni de facteurs de croissance dans le milieu de culture pour la croissance, ce par quoi ladite cellule CHO-K1 n'est pas modifiée par comparaison avec la lignée cellulaire ATCC CCL-61 de la cellule CHO-K1 déposée par l'introduction, la délétion ou l'inactivation d'un acide nucléique. L'invention concerne également un procédé pour obtenir ladite cellule CHO-K1 et sur un procédé pour la production d'un polypeptide hétérologue utilisant une telle cellule CHO-K1 selon l'invention.

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