Genetic incorporation of 3-aminotyrosine into reductases

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 5/10 (2006.01) C12Q 1/26 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2703736

This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.

Cette invention concerne des protéines de type réductase qui comprennent des résidus acides aminés NH2Y non naturels, des systèmes d'éléments orthogonaux pour incorporer des NH2Y dans des réductases et des procédés d'utilisation des résidus acides aminés NH2Y dans des réductases en tant que sondes moléculaires pour sonder la fonction, la structure et l'activité des réductases.

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