Particle-mediated transformation of woody plant species

Details Particle-mediated transformation of woody plant species Particle-mediated transformation of woody plant species

A - Human Necessities

01

H

A01H 5/00 (2006.01) A01H 5/10 (2006.01)

Patent

CA 2358475

The creation of transgenic trees is disclosed through the use of a particle-mediated transformation procedure. The method is demonstrated to be effective with various tissue types of poplar, including protoplasts, internode and petiole segments, and, most efficiently, with nodule cultures. Transgenic trees were recovered which express sufficient levels of the insect specific toxin from Bacillus thuringiensis so as to provide significant toxicity to feeding insects.

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