C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/01 (2006.01) C12N 15/10 (2006.01) C12N 15/85 (2006.01)
Patent
CA 2350976
This invention provides a method for selecting a clone of an ES cell containing a mutation in a gene that is expressed in a test cell comprising: (a) providing cDNA obtained by reverse transcription of mRNA of the test cell; (b) providing a collection of cultured ES cells organized into individual clones, wherein each clone is of an ES cell having a mutation in an exon in its genome, the mutation being in a different exon in cells of different clones; (c) providing an array of different single stranded polynucleotides, the polynucleotides being fragments of exons containing mutations in (b); (d) exposing the cDNA to the array under conditions permitting hybridization of polynucleotides in the array to nucleic acids; (e) detecting hybridization of cDNA to a polynucleotide on the array; and, (f) selecting a clone in the collection from which a hybridizing polynucleotide detected at (c) is an exon fragment. This invention also provides a system for testing expression of a gene in a test cell. Also provided is a preferred exon trap vector for mutating ES cells.
Smart & Biggar
University Of British Columbia
LandOfFree
Cell libraries indexed to nucleic acid microarrays does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cell libraries indexed to nucleic acid microarrays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell libraries indexed to nucleic acid microarrays will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1561281