C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07H 1/08 (2006.01) C07H 21/04 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2315257
The inventive method concerns the isolation and purification of short and long- chain nucleic acids from different biological and other starting materials and is characterized by the following steps: lysis of materials containing nucleic acids; incubation with a mineral supporting material; application on membranes having a pore size which is larger than the particle size of the supporting material; removal of the nucleic acids from the supporting material by elution, whereby the supporting material particles surprisingly remain on the membrane.
Le procédé selon l'invention permet d'isoler et de purifier des acides nucléiques à chaîne courte et à chaîne longue à partir de différents matériaux de départ biologiques ou d'une autre nature. Ce procédé se caractérise par le fait qu'il comprend les étapes suivantes: lyse de matériaux contenant des acides nucléiques; incubation avec un matériau support minéral; application sur des membranes présentant une taille de pore supérieure à la taille de particule du matériau support; et séparation des acides nucléiques du matériau support par élution, les particules de matériau support restant, de façon surprenante, sur la membrane.
Bendzko Peter
Hillebrand Timo
Deeth Williams Wall Llp
Invitek Gmbh
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