Oligonucleotide compositions and their use for the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) A61K 31/70 (2006.01) A61K 45/06 (2006.01)

Patent

CA 2483012

The present invention relates to 3'-OH, 5'-OH polynucleotide sequence compositions and methods for activating an immune response in an individual, and more preferably, for activating r antigen presenting cells in the individual. In one embodiment, the antigen presenting cell is a dendritic cell. The present invention also includes compositions and methods for activating dendritic cells in vitro. These dendritic cells may then be administered to an individual. Preferred 3'-OH, 5'-OH polynucleotide sequences comprise six bases wherein at least 50% of the bases are guanine and the 5' base is guanine. The compositions may comprise a phosphodiester or phosphorothioate backbone.

La présente invention a trait à des compositions de séquences polynucléotidiques 3'-OH, 5'-OH et des procédés permettant l'activation d'une réponse immunitaire chez un individu, et de préférence, l'activation de cellules présentatrices de l'antigène chez un individu. Dans un mode de réalisation, la cellule présentatrice de l'antigène est une cellule dendritique. La présente invention a trait également à des compositions et des procédés permettant l'activation de cellules dendritiques in vitro. Ces cellules dendritiques peuvent être administrées à un individu. Les séquences polynucléotidiques préférées 3'-OH, 5'-OH comprennent six bases dans lesquelles au moins 50 % des bases sont la guanine et la base 5' est la guanine. Les compositions peuvent comprendre un squelette phosphodiester ou phosphorothioate.

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