Method and device for determining an analyte

G - Physics – 01 – N

Patent

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G01N 33/558 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2358506

The invention relates to a method and a device for the differential determination of a quantity of at least one analyte in a liquid sample. A test strip which has at least two areas is used, these areas being composed in such a way that they can provide information about the quantity of the at least one analyte. The test strip also ensures that the sample or the portion of the sample flows laterally through the strip and the at least two areas by having a predetermined capacity for binding the at least one analyte in the first area. When this capacity is exceeded by a too great quantity of the at least one analyte, the at least one analyte is bound in at least one second area, the capacity of the second area being essentially equal to the capacity of the first area and the binding of the at least one analyte in the at least one first area and the at least one second area being measured by a detectable property.

L'invention concerne un procédé et un dispositif permettant la détermination différentielle d'une quantité d'au moins un analyte contenu dans un échantillon liquide, cela au moyen d'une bande test qui comporte au moins deux zones dont la constitution leur permet de donner une information sur la quantité du ou des analytes, la bande test garantissant un écoulement latéral traversant de l'échantillon ou d'une fraction d'échantillon et garantissant la présence des zones qui sont au moins au nombre de deux. Selon l'invention, une première zone présente une capacité prédéfinie à fixer le ou les analytes et, en cas de dépassement de cette capacité par la quantité trop importante du ou des analytes pour cette capacité, il se produit une liaison du ou des analytes dans au moins une seconde zone, la capacité de la seconde zone étant sensiblement la même que celle de la première zone, et la fixation du ou des analytes dans la première et la seconde zone est mesurée en fonction d'une caractéristique détectable.

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