Vector to produce biologically important peptides

Details Vector to produce biologically important peptides Vector to produce biologically important peptides

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/70 (2006.01) C07K 7/14 (2006.01) C07K 14/245 (2006.01) C07K 14/62 (2006.01) C12N 1/21 (2006.01) C12N 15/16 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2034385

Abstract In this patent application we have described the construction of a novel secretion vector based on E.coli enterotoxin coding sequence. We have shown categorically that pre and pro region of toxin gene are absolutely necessary for extra cellular secretion of the stable toxin. We have also shown with specific examples that when the nucleotide coding sequence of a heterologous peptide is fused in frame to the end of the pro region in the st gene, the resultant vector in an E.coli host secretes extracellularly correctly processed heterologous peptide. This application also includes construction of suitable vectors where this gene fusion can be achieved. General methods to create such fusions involving a) recombinant DNA technology and b) the use of site directed in vitro mutagenesis, have also been described. A general method of purification of heterologous peptides is also described in this application. This novel vector system can be used for hyperproduction and extracellular secretion of peptides of biological importance.

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