Enzyme-catalyzed in vitro modification and synthesis of...

Details Enzyme-catalyzed in vitro modification and synthesis of... Enzyme-catalyzed in vitro modification and synthesis of...

C - Chemistry, Metallurgy

12

N

C12N 13/00 (2006.01) C12N 15/00 (2006.01) C12P 19/34 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2491629

The present invention relates to a simple, efficient, and accelerated method for enzyme-catalyzed in vitro modifica-tion and synthesis of nucleic acid using uninterrupted and brief microwave irradiation at a frequency ranging between 2300 to 2500 MHz, with the power output ranging between 600 to 900 watts, and for a period ranging from 5 to 120 seconds; and further, an apparatus for using said method.

L'invention concerne un procédé simple, efficace et accéléré de modification in vitro catalysée par une enzyme et de synthèse d'acide nucléique utilisant une irradiation par hyperfréquences brève et ininterrompue, à une fréquence comprise entre 2300 et 2500 MHz, la puissance de sortie se situant dans la plage comprise entre 600 et 900 watts, pendant une durée comprise entre 5 et 120 secondes ; et un appareil utilisant ce procédé.

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