Human micrornas and methods for inhibiting same

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/04 (2006.01) C07H 21/02 (2006.01) C07K 14/00 (2006.01) C07K 17/00 (2006.01) C12N 15/11 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2605701

The invention relates to isolated DNA or RNA molecules comprising at least ten contiguous bases having a sequence in a microRNA shown in SEQ ID NOs: 1-94; 281-374; 467- 481 ; 497-522; or 549, except that up to thirty percent of the bases may be wobble bases, and up to 10% of the contiguous bases may be non- complementary. The invention further relates to modified single stranded microRNA molecules, isolated single stranded anti-microRNA molecules and isolated microRNP molecules. In another embodiment, the invention relates to a method for inhibiting microRNP activity in a cell.

L'invention concerne des molécules d'ADN et d'ARN isolées comprenant au moins dix bases contiguës comportant une séquence dans un micro-ARN représentée par les numéros d'identification SEQ ID NO : 1-94 ; 281-374 ; 467-481 ; 497-522 ; ou 549, excepté que jusqu'à 30 % des bases peuvent être des bases Wobble et jusqu'à 10 % des bases contiguës peuvent être non complémentaires. L'invention concerne en outre des molécules de micro-ARN à simple brin modifiées, des molécules d'anti-micro-ARN à simple brin isolées et des molécules de micro-RNP isolées. Dans un autre mode de réalisation, l'invention concerne un procédé pour inhiber l'activité micro-RNP dans une cellule.

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