Preparation and storage of pancreatic islets

Details Preparation and storage of pancreatic islets Preparation and storage of pancreatic islets

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) A01N 1/02 (2006.01) A61K 35/39 (2006.01) C12M 3/08 (2006.01) C12N 5/06 (2006.01) C12S 3/24 (2006.01)

Patent

CA 2179101

A method, a solution and a chamber for the preparation and storage of pancreatic islets. The method comprising contacting a pancreas with a warm collagenase solution, digesting the pancreas in the warm collagenase solution to form warm digest, adding cold preservative solution to the warm digest, agitating the warm digest/cold preservative solution at a temperature between about 0° and 15°C, to thereby further digest the partially digested pancreas included in the warm digest, to form cold digest and collecting liquid from the cold digest to form isolated islets. The cold preservative solution and a pancreatic islet preservative solution of the present invention comprise D-mannitol, K-lactobionate and a buffer.

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