Gene constructs encoding crop-protecting agents, as well as...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) A01N 63/02 (2006.01) A61K 47/48 (2006.01) C07K 14/245 (2006.01) C07K 14/325 (2006.01) C07K 16/14 (2006.01) C07K 16/18 (2006.01) C07K 19/00 (2006.01) C12N 9/24 (2006.01) C12N 15/31 (2006.01) C12N 15/62 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2200434

The invention provides gene constructs comprising a nucleotide sequence encoding an antibody or part thereof which specifically binds to structures of a plague organism or pathogen, and a nucleotide encoding a protein which is toxic to said organism. The toxic protein may be a toxin or an enzyme having a toxic function. If the pathogen is a fungus or a bacterium, said antibody may be specific to cell-wall or cell-membrane antigens of the pathogen; if the plague organism is an insect or a nematode, said antibody may be specific to antigens of the alimentary tract. Also provided are expression systems and plague-resistant plants transformed with these systems, as well as immunotoxins encoded by the gene constructs and organisms containing the constructs or the immunotoxins for use as external crop-protecting agents.

Produits d'assemblage géniques comportant une séquence nucléotidique codant un anticorps ou une partie de celui-ci qui se lie de manière spécifique aux structures d'un agent pathogène ou organisme pesteux, et un nucléotide codant une protéine toxique vis-à-vis de cet organisme. La protéine toxique peut être une toxine ou une enzyme à fonction toxique. Si l'agent pathogène est un champignon ou une bactérie, ledit anticorps peut être spécifique des antigènes de membrane ou paroi cellulaire de l'agent pathogène. Si l'organisme pesteux est un insecte ou un nématode, ledit anticorps peut être spécifique des antigènes du tube digestif. On a également prévu des systèmes d'expression et des plantes résistantes à la peste transformées à l'aide de ces systèmes, ainsi que des immunotoxines codées par les produits d'assemblage géniques, et des organismes renfermant les produits d'assemblage ou les immunotoxines et servant d'agents externes de protection des cultures.

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