Methods for assembly of high fidelity synthetic polynucleotides

Details Methods for assembly of high fidelity synthetic polynucleotides Methods for assembly of high fidelity synthetic polynucleotides

C - Chemistry, Metallurgy

40

B

C40B 40/06 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C40B 50/06 (2006.01) C40B 50/14 (2006.01)

Patent

CA 2584984

Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of nucleic acid polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre~amplification of one or more oligonucleotides using "universal" primers, reduction of the error rate in oligonucleotide and/or nucleic acid products, and sequence optimization and oligonucleotides design. Also provided are low-purity arrays of nucleic acids and methods for assembling nucleic acids using oligonucleotides obtained from low-purity arrays.

L'invention concerne des procédés de fabrication d'ADN synthétiques, soit des ADN obtenus, du moins en grande partie, par synthèse chimique de polymères d'acides nucléiques. L'invention concerne également des procédés permettant d'assembler plusieurs ADN dans le même stock par assemblage multiplexé d'oligonucléotides synthétiques. Dans des modes de réalisation exemplaires, les procédés impliquent la préamplification d'au moins un oligonucléotide au moyen d'amorceurs "universels", la réduction du taux d'erreur dans des produits d'oligonucléotides et/ou d'acides nucléiques, et l'optimisation séquentielle et la conception d'oligonucléotides. L'invention concerne enfin des réseaux d'acides nucléiques de faible pureté ainsi que des procédés d'assemblage d'acides nucléiques au moyen d'oligonucléotides obtenus de réseaux de faible pureté.

Affiliated with

Also associated with

Rating

Methods for assembly of high fidelity synthetic polynucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for assembly of high fidelity synthetic polynucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for assembly of high fidelity synthetic polynucleotides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1570799