Oligonucleotides and uses thereof

Details Oligonucleotides and uses thereof Oligonucleotides and uses thereof

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2703227

A method for determining the number of tandem repeats in a target polynucleotide, the method comprising (a) providing a sample containing the target polynucleotide, wherein one or more of the tandem repeats in the target polynucleotide is in single stranded form, (b) hybridising a labelled probe oligonucleotide to the single stranded portion of the target polynucleotide, wherein the probe oligonucleotide is complementary to at least one of the tandem repeats, and at least 5 nucleotides of the probe oligonucleotide are complementary to the tandem repeats, in the single stranded portion of the target polynucleotide, and (c) determining the number of tandem repeats in the target polynucleotide based on the hybridisation of the probe oligonucleotide to the single stranded portion of the target polynucleotide.

L'invention porte sur un procédé destiné à déterminer le nombre de répétitions en tandem d'un polynucléotide cible. Le procédé consiste à (a) se procurer un échantillon contenant le polynucléotide cible, une ou plusieurs des répétitions en tandem du polynucléotide cible étant sous forme à brin unique; (b) hybrider un oligonucléotide sonde marqué sur le brin unique du polynucléotide cible, l'oligonucléotide sonde étant complémentaire d'au moins l'une des répétitions en tandem et au moins 5 de ses nucléotides étant complémentaires des répétitions en tandem sur le brin unique du polynucléotide cible; et (c) déterminer le nombre de répétitions en tandem du polynucléotide cible selon l'hybridation de l'oligonucléotide sonde sur le brin unique du polynucléotide cible.

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