Fermentation process for creatinine iminohydrolase

Details Fermentation process for creatinine iminohydrolase Fermentation process for creatinine iminohydrolase

C - Chemistry, Metallurgy

12

N

195/123, 195/43

C12N 9/78 (2006.01) C12N 1/38 (2006.01)

Patent

CA 2022825

-0- FERMENTATION PROCESS FOR CREATININE IMINOHYDROLASE Abstract of the Disclosure A fermentation process and improved aqueous nutrient medium are used for the production of urease-free creatinine iminohydrolase from an aerobic soil microorganism. In the process, an inoculum culture of the microorganism is transferred into a production medium to generate microorganisms in which creatinine iminohydrolase production has been induced, said nutrient medium containing a source of ammonia and after the ammonia is substantially exhausted, incrementally adding a solution of creatinine, controlling the pH by addition of .alpha.-ketoglutaric acid, and extracting urease-free creatinine iminohydrolase. An improved aqueous nutrient medium for use as the production medium is disclosed.

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