Method and apparatus for electrophoretic analysis

G - Physics – 01 – N

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G01N 27/447 (2006.01) B01D 57/02 (2006.01) C12Q 1/68 (2006.01) G01N 21/76 (2006.01) G01N 21/77 (2006.01)

Patent

CA 2187780

In a method and system for electrophoretic analysis of fluorophore-labelled substance mixtures, an electrophoresis zone (I) having at least one lane (3), and photometric detector means (4) fixed relative to this lane for detecting separated fluorophore-labelled substances as they pass the detector means (4) are used. According to the invention, there are further used means (5, 8, 9, 10) for alternately emitting to the electrophoresis lane light of different wavelengths or wavelength bands (.lambda.1, .lambda.2) corresponding to the excitation wavelengths for two or more different fluorophores, and synchronisation means for relating a detected fluorescent light emission to a respective excitation wavelength.

Divulgation d'une méthode et d'un système d'analyse électrophorétique de mélanges de substances marquées au fluorogène. Le système comporte une cuve à électrophorèse (1) comptant au moins un canal (3) et un photomètre (4) fixe par rapport à ce canal pour détecter des substances distinctes marquées au fluorogène à leur passage devant le photomètre (4). Selon l'invention, d'autres dispositifs sont utilisés (5, 8, 9 et 10) pour émettre en alternance dans le canal de la lumière de longueurs d'ondes ou de bandes de longueurs d'ondes différentes (.lambda.1, lambda.2) correspondant aux longueurs d'ondes d'excitation pour au moins deux fluorogènes différents, ainsi que des dispositifs de synchronisation pour rattacher une émission de lumière fluorescente détectée à une longueur d'ondes d'excitation correspondante.

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