Recombinant chondroitinase abc i and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/88 (2006.01)

Patent

CA 2558984

The invention relates to chondroitinase ABC I and uses thereof. In particular, the invention relates to recombinant and modified chondroitinase ABC I, their production and their uses. The chondroitinase ABC I enzymes of the invention are useful for a variety of purposes, including degrading and analyzing polysaccharides such as glycosaminoglycans (GAGs). These GAGs can include chondroitin sulfate, dermatan sulfate, unsulfated chondroitin and hyaluronan. The chondroitinase ABC I enzymes can also be used in therapeutic methods such as promoting nerve regeneration, promoting stroke recovery, treating spinal cord injury, treating epithelial disease, treating infections and treating cancer.

La présente invention a trait à la chondroïtinase ABC I et son utilisation. En particulier, l'invention a trait aux enzymes de la chondroïtinase ABC I recombinante et modifiée, leur production et leurs utilisations. Les enzymes de chondroïtinase ABC I de l'invention sont utiles pour une variété d'applications, comprenant la dégradation et l'analyse de polysaccharides tels que les glycoaminoglycannes (GAGs). Ces GAGs peuvent comporter du sulfate de chondroïtine, du sulfate de dermatanne, et de la chondroïtine et de hyaluronan non sulfaté(e). Les enzymes de la chondroïtinase ABC I peuvent également être utilisées dans des procédés thérapeutiques tels que la promotion de la régénération nerveuse, la promotion du rétablissement suite à un accident vasculaire cérébral, le traitement de la lésion de la moelle épinière, le traitement de maladie épithéliale, le traitement d'infections et le traitement du cancer.

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