Methods for retrotransposing long interspersed elements (lines)

Details Methods for retrotransposing long interspersed elements (lines) Methods for retrotransposing long interspersed elements (lines)

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 7/01 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2474810

It is intended to provide a method of LINE retro-position. Namely, a method which comprises transferring an RNA containing a 3~ URT fragment of a LINE in cells and then trans-positioning the ORF protein of the LINE to thereby retro- transposition the RNA. A method of modifying a retro-transposition target site of a LINE by substituting the endonuclease domain of the LINE by the endonuclease domain of another LINE. This LINE retro-transposition method is useful in transferring a novel gene.

Méthode de rétroposition de séquences LINE qui consiste à transférer un ARN contenant un fragment 3' URT d'une séquence LINE dans des cellules, puis à opérer la transposition de la protéine ORF de la séquence LINE de manière à obtenir ainsi la rétro-transposition de l'ARN. La présente invention concerne encore une méthode de modification d'un site cible de rétro-transposition d'une séquence LINE par substitution du domaine d'endonucléase de la séquence LINE par le domaine d'endonucléase d'une autre séquence LINE. Cette méthode de rétro-transposition de séquence LINE est utile pour transférer un nouveau gène.

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