C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/02 (2006.01) C12Q 1/24 (2006.01)
Patent
CA 2329842
A method and an apparatus for determining the concentration at which a growth-altering agent has an appreciable effect on the growth of a target microorganism are provided. The method comprises the steps of (a) providing a microorganism growth medium; (b) providing a sensible reagent, which includes a growth-altering agent mixed with a marker that has a signal with a magnitude proportional to the concentration of the marker; (c) incorporating the sensible reagent into the growth medium, in a manner that creates a gradient of growth-altering agent and marker concentrations within the growth medium; (d) inoculating the growth medium with the target microorganism; (e) incubating the inoculated growth medium for a period of time sufficient for the target microorganism to grow a detectable amount; (f) evaluating growth characteristics of the microorganism in a region containing the growth-altering agent; (g) measuring the magnitude of the marker signal in that region; and (h) determining the concentration of the growth-altering agent using the measured magnitude of the marker signal.
Levine Robert A.
Riches Mckenzie & Herbert Llp
Wardlaw Stephen C.
Wardlaw Partners Lp
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