Regulated vectors for controlling dna hypermutability in...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2476559

The present invention features mammalian expression vectors that are useful for controlling DNA hypermutability in mammalian cell as well as the encoding polynucleotide sequences of vector sequences. In related aspects the invention features expression vectors and host cells comprising such polynucleotides. In other related aspects, the invention features transgenic cells expressing a mutator gene to enhance genome-wide mutagenesis, due to, for example, the presence of an exogenous mutator-encoding polynucleotide sequence. Further, the invention provides methods for using vector sequences that can remove the expression of such gene to restore DNA stability in a host cell.

L'invention concerne des vecteurs d'expression de mammifères utiles dans la régulation de l'hypermutabilité d'ADN dans des cellules de mammifères, ainsi que des séquences de polynucléotides codant pour ces séquences de vecteur. Dans des modes de réalisation associés, l'invention concerne des vecteurs d'expression et des cellules hôtes contenant ces polynucléotides. Dans d'autres modes de réalisation, elle concerne des cellules transgéniques exprimant un gène mutateur afin d'augmenter la mutagénèse du génome dans son ensemble, due, par exemple, à la présence d'une séquence de polynucléotides exogène codant pour le gène mutateur. Elle concerne enfin des procédés d'utilisation des séquences de vecteur qui peuvent supprimer l'expression d'un tel gène afin de rétablir la stabilité de l'ADN dans une cellule hôte.

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