Method and test kits for detection of bacteriophage

Details Method and test kits for detection of bacteriophage Method and test kits for detection of bacteriophage

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C12Q 1/04 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2169168

A method for detection, identification and/or quantification of bacteriophage of bacterial host specificity for bacterial genus, species or serotype, based upon the occurrence of release of cell contents, particularly nucleotides e.g. ATP, on lysis of bacterial cell walls on incubation with bacterial host cells. When new phage particles are released at the end of the phage replication cycle nucleotide levels are measured and compared with controls. The method provides for the detection of specific phages which is faster and more sensitive than known techniques. The method is only limited by the availability of host bacteria/target phage pairings.

L'invention concerne un procédé de détection, d'identification et/ou de quantification de bactériophages à spécificité d'hôte bactérien pour un genre, une espèce ou un sérotype bactérien, ledit procédé étant basé sur la libération du contenu des cellules, notamment les nucléotides comme par exemple l'adénosine triphosphate, sur la lyse des parois de cellules bactériennes lors de l'incubation avec des cellules hôtes bactériennes. Lorsque de nouvelles particules de phages sont libérées à la fin du cycle de réplication des phages, on mesure les niveaux de nucléotides et on les compare avec des témoins. Ce procédé permettant de détecter des phages spécifiques est plus rapide et plus sensible que les techniques connues; il est uniquement limité par la disponibilité des appariements bactérie hôte/phage cible.

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