G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/50 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2440733
An in vitro method for diagnosing a contact allergy in a subject comprises: (i) providing (a) a contact allergen; (b) T-cells from said subject; and (c) a surface to which is immobilised a first specific binding agent capable of specifically binding to a cytokine released from a T-cell in response to said allergen; (ii) contacting said sample with said allergen under conditions which are suitable for (a) release of said cytokine by T cells that have been presensitised in vivo to said allergen; and (b) binding of said cytokine to said first specific binding agent; and (iii) detecting binding of said substance to said first specific binding agent. A kit which enables the method to be carried out is also described.
L'invention concerne une méthode in vitro permettant de diagnostiquer une allergie de contact chez un sujet. Cette méthode consiste: (i) à fournir (a) un allergène de contact, (b) des lymphocytes T provenant dudit sujet, et (c) une surface sur laquelle est immobilisé un premier agent de liaison spécifique pouvant se lier de manière spécifique à une cytokine libérée par un lymphocyte T en réponse audit allergène; (ii) à mettre en contact ledit échantillon avec ledit allergène, dans des conditions appropriées (a) à la libération de ladite cytokine par des lymphocytes T qui ont été présensibilisés in vivo audit allergène, et (b) à la liaison de ladite cytokine audit agent de liaison spécifique; et (iii) à détecter la liaison de ladite substance audit premier agent de liaison spécifique. L'invention concerne également un kit permettant de mettre en oeuvre ce procédé.
Fetherstonhaugh & Co.
Mabtech Ab
LandOfFree
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